Composite

Part:BBa_K1969013:Design

Designed by: Yijie Lai   Group: iGEM16_SJTU-BioX-Shanghai   (2016-10-10)


Nano-lantern(cAMP-1.6) regulated by GAL1 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1818
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 70
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1096
    Illegal BsaI.rc site found at 1680
    Illegal SapI.rc site found at 1881


Design Notes

Use a inducible promoter GAL1 to mediate the expression of reporter protein Nano-lantern(cAMP-1.6).


Source

The expression of Nano-lantern(cAMP-1.6) may affect the sensing resolution of intracellular cAMP level, so creating expression cassettes with different expression level is critical for the project. This cassette is one of median expression level.

References